UTF-8
An Advanced Technique: Fluorescent Tags in DNA Chips
An Advanced Technique: Fluorescent Tags in DNA Chips
p. 7 of 7
An important application of fluorescence is in DNA chips, also called DNA microarrays. A DNA chip is an arrangement of nucleotides with known sequences printed on a solid surface using a technology such as photolithography. These chips can be used to measure the expression of large number of genes simultaneously.
A DNA microarray
Fluorescent tags are attached to single strands of the DNA sample an investigator wants to understand. The sample is then exposed to known DNA sequences on a chip. They attach to their complements, and fluoresce when placed under the light of a scanner. The position of the resulting fluorescence will tell the scientist which genes are there. The following model schematically illustrates the fluorescence process of tags hybridized, or attached, to certain positions in a chip:
Note: In the above model, we just show a single DNA strand on each address of the array (i.e. each site on the array). In reality, each address can contain many DNA strands of the same sequence.
< Previous>
Attention, authors! If you are interested in modifying this activity, please click the link below to download it:
<< Download this activity >>
1
4.0
true14
14
true14
14
1
14
6.0
14
6.0
13
14
6.0
1
14
6.0
true14
6.0
6.0
14
6.0
11
6.0
1
org.concord.modeler.PageSlider
0
0.9999999983443153
0.10000000149011612
1.0
18
Simulation speed
200
70
false
true
{value="0.9999999983443153", label="Fast"}{value="0.09999999983443153", label="Slow"}
Simulation speed of light-matter interactions
11
1
org.concord.mw2d.activity.AtomContainer
fluorescence7$0.mmlfalse
1
org.concord.modeler.PagePhotonSpectrometer
0
0.0
15.0
20
55030
1
spectrum_plus.jpg
40.0
11
6.0
1
6.0
2.0
1
2.0
org.concord.modeler.text.LineIcon0.95false-1000000
100.0
100.0
true11
11
6.0
2.0
1
2.0
org.concord.modeler.text.LineIcon0.95false-1000000
11
6.0
11
6.0
1
org.concord.modeler.ActivityButton
Create a report
Fluorescene Activity
fluorescence1.cml, fluorescence2.cml, fluorescence3.cml, fluorescence4.cml, fluorescence5.cml, fluorescence6.cml, fluorescence7.cml, fluorescence8.cml
Raised Bevel
Create a report for a page group in a new window
6.0
1
11
6.0
1
11
fluorescence7.cml11
11
6.0
11
6.0
2.0
1
2.0
org.concord.modeler.text.LineIcon0.95false-1000000
6.0
1
true
6.0
1
6.0
1
true../../activityzip/fluorescence.zip16
true16
6.0
1
6.0
2.0
1
2.0
org.concord.modeler.text.LineIcon0.95false-1000000