UTF-8
Protein Conservation 5
255229185
Protein Folding & Amino Acid Substitutions
page 6 of 11
1
true18true
1
18true
1
1
org.concord.modeler.PageTextBox
710.0
163.0
<html>
<head>
</head>
<body marginwidth="10" marginheight="10">
<font face="verdana, arial, helvetica, sans-serif" size="5">What happens
when a mutation in a gene causes a change in the amino acid sequence of a
protein? The <i>substitution</i> of one amino acid for another can change
the structure of the protein to some degree. In a short protein chain like
the one we are using to demonstrate these principles, a single amino acid
substitution can have a dramatic effect on folding.</font>
</body>
</html>
-392113
Raised Bevel
1
1
org.concord.mw2d.activity.AtomContainer
protein_conservation5$0.mmlfalsetrue
org.concord.modeler.PageTextBox
350.0
292.0
<html>
<head>
</head>
<body marginwidth="10" marginheight="10">
<strong><font face="verdana, arial, helvetica, sans-serif" size="4">Effects
of Mutations on Folding</font></strong>
<ul>
<li>
<font face="verdana, arial, helvetica, sans-serif" size="4">Click run
to see the peptide fold. </font>
</li>
<li>
<font face="verdana, arial, helvetica, sans-serif" size="4">Use the
menu below to select a mutation. Each mutation changes the highlighted
amino acid <img src="highlightedAA.gif"></font>
</li>
<li>
<font face="verdana, arial, helvetica, sans-serif" size="4">Run the
model and notice if the different amino acid affects folding.</font>
</li>
<li>
<font face="verdana, arial, helvetica, sans-serif" size="4">Take a
snapshot of each folded peptide. Use the "Snapshots" button in the
tool bar at the top of this page to refer to your snapshots as you
answer the questions below. </font>
</li>
</ul>
</body>
</html>
-392113
Raised Bevel
1
org.concord.modeler.PageComboBox
org.concord.mw2d.models.MolecularModel
0
Execute MW script
Mutate an amino acid
org.concord.modeler.PageButton
Take a snapshot
false
org.concord.mw2d.models.MolecularModel
0
Take a snapshot
org.concord.modeler.PageTextBox
270.0
20.0
<html>
<head>
</head>
<body>
</body>
</html>
false
1
1
1
org.concord.modeler.PageTextArea
660
179
<html>
<head>
</head>
<body>
<b>Compared to the original protein without a mutation, how does each of
the three different mutated peptides fold? Make sure to say where in each
folded peptide the mutated (highlighted) amino acid ends up. </b>
</body>
</html>
-1a47
1
1
1
org.concord.modeler.PageTextArea
660
179
<html>
<head>
</head>
<body>
<b>Recall that water is polar. Each mutation you looked at changed the
side chain of the highlighted amino acid. One was polar, one charged
(charged amino acids are even more strongly polar) and one was
hydrophobic. For each mutation, explain the reason that the protein either
folded differently, or folded the same way as the original protein.
Remember to use words such as "polar", "non-polar", "hydrophobic", and
"hydrophilic" </b>
</body>
</html>
-1a47
1
1
org.concord.modeler.text.LineIcon750.01-1000000
1
1
protein_conservation4a.cmlupBackSmall.gif
0 26 70
protConsIndex.cmlupContents.gif
protein_conservation6.cmlupArrowSmall.gif
protein_conservation6.cml
1
0 26 70